Journal: Viruses

Article Title: Infectious Spleen and Kidney Necrosis Virus Triggers Ferroptosis in CPB Cells to Enhance Virus Replication.

doi: 10.3390/v17050713

Figure Lengend Snippet: Figure 3. ISKNV infection induces ferroptosis in CPB cells. (A) Transmission electron microscopy of CPB cells treated with DMSO (72 h), erastin (10 µmol/L, 72 h), and ISKNV (100 MOI, 24 h, 48 h, 72 h). Scale bars = 1 µm. (B) Analysis of Fe2+ levels in CPB cells after treatment with ISKNV (100 MOI) using the fluorescent probe FerroOrange by laser scanning confocal microscopy. Scale bars = 20 µm. (C) Quantitative analysis of the mean fluorescence intensity of (B) using Image J. (D) Analysis of intracellular ROS levels using DCFH-DA staining, and laser scanning confocal microscopy of CPB cells treated with ISKNV (100 MOI) for 24–72 h. Scale bars = 10 µm. (E) Quantitative analysis of the mean fluorescence intensity of (D) using Image J. (F–H) Detection of Fe2+, ROS, and MDA levels in cell lysates treated with ISKNV (100 MOI) for 24–72 h by microplate reader. * p < 0.05, ** p < 0.01, and *** p < 0.001, with p > 0.05 considered not significant (ns).

Article Snippet: The Fe2+ content of the cells was detected by laser scanning confocal microscopy and a microplate reader using the fluorescent probe FerroOrange (Elabscience, E-BC-F101, Wuhan, China).

Techniques: Infection, Transmission Assay, Electron Microscopy, Confocal Microscopy, Fluorescence, Staining

Journal: Discover Oncology

Article Title: Novel N 6 -methyladenosine (m 6 A) writer METTL16 promotes the cervical cancer tumorigenesis by targeting FTH1-dependent ferroptosis

doi: 10.1007/s12672-026-04403-8

Figure Lengend Snippet: METTL16 inhibited the ferroptosis of cervical cancer cells. A Transmission electron microscopy (TEM) was performed to detect the cellular substructures transformation in cervical cancer cells with Erastin (10 µmol/L). HeLa cells were transfected with overexpression of METTL16 plasmids (ov-NC, ov-METTL16). CaSki cells were transfected with silencing of METTL16 plasmids (sh-NC, sh-METTL16-1, sh-METTL16-2). B The Fe 2+ level in cervical cancer cells was tested by Iron Colorimetric Assay Kit. C MDA was tested in cervical cancer cells. D GSH was tested in cervical cancer cells. E , F FerroOrange fluorescent assay was performed to test the Fe 2+ in cancer cells. * p < 0.05, ** p < 0.01

Article Snippet: FerroOrange fluorescent assay was performed to test the Fe 2+ in cancer cells FerroOrange fluorescent probe (Servicebio, Cat. G1727).

Techniques: Transmission Assay, Electron Microscopy, Transformation Assay, Transfection, Over Expression, Colorimetric Assay, Fluorescence

Journal: Science Advances

Article Title: Macrophage-targeted Mms6 mRNA-lipid nanoparticles promote locomotor functional recovery after traumatic spinal cord injury in mice

doi: 10.1126/sciadv.ads2295

Figure Lengend Snippet: ( A ) Schematics of the experimental workflow. SCI mice were received a single injection of Mms6 mRNA-LNPs. ( B and C ) Basso Mouse Scale (BMS) scores and subscores were assessed in SCI mice ( n = 6 mice per group). * P < 0.05 and ** P < 0.01. ( D ) Representative footprint images at 28 dpi of SCI. The forelimbs and hindlimbs were painted in black and red, respectively. ( E ) Evaluation of stride length using footprint images ( n = 6 mice per group). ** P < 0.01 versus Sham group; ## P < 0.01 versus empty LNP group; && P < 0.01 versus Mms6 mRNA-LNP (0.5 mg/kg) group; $ P < 0.05 versus Mms6 mRNA-LNP (1 mg/kg) group. ( F ) Statistical analysis of flow cytometry results showing the percentage of FerroOrange positive Mφ ( n = 3 mice per group). ** P < 0.01 versus empty LNP group. ( G ) Statistical analysis of flow cytometry results showing the percentage of Liperfluo positive Mφ ( n = 3 mice per group). ** P < 0.01 versus empty LNP group. dpi, days post-injury.

Article Snippet: Intracellular ferrous ions and lipid peroxides in macrophages were detected using FerroOrange and Liperfluo fluorescent probes, respectively (Dojindo, Shanghai, China).

Techniques: Injection, Flow Cytometry